Glen research/C8-TMS-Alkyne-dT-CE Phosphoramidite/1kit/10-1545-02E
商品编号：
10-1545-02E
品牌：
Glenresearch
市场价：
¥21000.00
美元价：
12600.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Technical Documents
Description
Details
Intellectual Property
Dilution/Coupling Data
References
Technical Documents
Description
5-Ethynyl-dU offers convenient click conjugation with an azide to generate a label rigidly attached to one of the oligonucleotide bases. 5-Ethynyl-dU is subject to base-catalyzed hydration during cleavage and deprotection, especially when using a strong base or heat.Hydration of an ethynyl group forms a methyl ketone which subsequently blocks potential click reactions.Mild deprotection conditions are necessary when using 5-Ethynyl-dU-CE Phosphoramidite to prevent this side reaction.TIPS-5-Ethynyl-dU-CE Phosphoramidite, containing a protected alkyne, offers broader compatibility with oligonucleotide synthesis and deprotection.Protecting the 5-ethynyl group with a triisopropylsilyl (TIPS) protecting group prevents acid or base catalyzed hydration during oligonucleotide synthesis and workup.A quick treatment with TBAF removes the TIPS protecting group.
Details
Usage
- Coupling: 3 minute coupling time recommended.
- Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.This is more than sufficient to completely remove the TMS protecting group.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Freezer storage, -10 to -30�C, dry |
| Stability | 1-2 days |
Intellectual Property
baseclick GmbH has been granted the following patents (1-3) besides its further patent applications (4-5). |1. WO 2006/117161 (New labelling strategies for the sensitive detection of analytes)|2. WO 2008/952775 (Click chemistry for the production of reporter molecules)|3. WO 2010/115957 (Click Chemistry on heterogeneous catalysts)|4. PCT/EP 2013/064610 (Anandamide-modified nucleic molecules)|5. PCT/EP 2015/056007 (Self-assembly of DNA Origami: a diagnostic tool)|baseclick GmbH holds a worldwide exclusive license for granted patent application|WO 03/101972 (Copper-catalysed ligation of azides and acetylenes for the nucleic acid field) in the area of diagnostics and research. As Glen Research and baseclick are partners, Glen Research is now able to help in sublicensing this outstanding technology.
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1545-02 | 0.25 g | .25grams | 2.76 | 78.67 | 47.2 | 29.5 | 21.45 | 15.73 | 3.93 |
| 10-1545-90 | 100 µmol | .091grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1545-95 | 50 µmol | .045grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1545-02 | 0.25 g | .25grams | 4.11 | 0.07 | 75.8 | 47.38 | 34.45 | 4.74 |
| 10-1545-90 | 100 µmol | .091grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
| 10-1545-95 | 50 µmol | .045grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
References
REFERENCE(S)| 1 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064; V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.| 2 C. J. Burrows, J. G. Muller, Chem. Rev. 1998, 98, 1109 â 1151.| 3 T. R. Chan, R. Hilgraf, K. B. Sharpless, V. V. Fokin, Org. Lett. 2004, 6, 2853 â 2855.| 4 J. Gierlich, G. A. Burley, P. M. E. Gramlich, D. M. Hammond, T. Carell, Org. Lett. 2006, 8, 3639-3642. F. Seela, V. R. Sirivolu, Chem. Biodiversity 2006, 3, 509-514.| 5 P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, Angew. Chem. 2008, 120, 3491â3493; Angew. Chem. Int. Ed. 2008, 47, 3442â 3444.| 6 P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chem. Int. Ed. 2008, 47, 8350-8358.
Related Products
Related Products
C8-Alkyne-dT-CE PhosphoramiditeCatalog #: 10-1540
C8-TIPS-Alkyne-dT-CE PhosphoramiditeCatalog #: 10-1544
C8-Alkyne-dC-CE PhosphoramiditeCatalog #: 10-1543
C8-TMS-Alkyne-dC-CE PhosphoramiditeCatalog #: 10-1542
C8-TIPS-Alkyne-dC-CE PhosphoramiditeCatalog #: 10-1541
品牌介绍
Glen research对我们的员工，我们的客户和环境的承诺Maravai LifeSciences致力于在所有业务活动中成为环境，健康与安全（EHS）管理方面的领导者，符合Maravai LifeSciences的商业利益和行为准则。我们承诺：雇员我们将通过提供零伤害的工作场所来预防伤害和疾病并促进健康的生活方式，从而对每位员工产生积极影响。我们将确保我们的员工具有执行此政策的意识，工具和知识。可持续发展我们将通过使用确保安全，效率和社会责任的商业惯例，以现在和将来保护我们的员工，社区，股东和环境的方式，进行可持续思考和采取行动。合规我们将遵守适用的法律和法规，同时提供程序和程序以确保我们的遵守。文化我们将通过停工授权，查明根本原因并采取纠正措施，倡导一种不容忍不安全状况和行为的文化。业务整合我们会将环境，健康和安全考虑因素纳入我们的业务活动。顾客我们将与客户紧密合作，以帮助他们满足其环境，健康和安全需求。供应商和承包商我们将与我们的供应商和承包商合作，以改善环境，健康和安全绩效。社区与政府我们将参与社区和政府的环境，健康和安全计划。这些价值观得到了Maravai高层管理人员的重视，并在组织内的所有责任级别得到强调。卡尔·赫尔（Carl Hull）首席执行官
联络我们
