4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:亚磷酰安,DNA和RNA寡核苷酸的化学合成物
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Electrophysiological > 格伦研究院/dA Me磷酰胺/1kit/10-1100-02E
商品详细格伦研究院/dA Me磷酰胺/1kit/10-1100-02E
格伦研究院/dA Me磷酰胺/1kit/10-1100-02E
格伦研究院/dA Me磷酰胺/1kit/10-1100-02E
商品编号: 10-1100-02E
品牌: Glenresearch
市场价: ¥4500.00
美元价: 2700.00
产地: 美国(厂家直采)
公司:
产品分类: 电生理设备
公司分类: Electrophysiological
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Technical Documents
Description
Details
Dilution/Coupling Data
Related Products

Technical Documents

Safety Data Sheet

Synthesis Using Methyl Phosphoramidites

Glen Report 9.12: Protecting Groups for DNA, RNA and 2"-OMe-RNA Monomers


Description

Methyl Phosphonamidites may be used in DNA synthesizers following conventional CE Phosphoramidite protocols to produce oligonucleotides containing one or more methyl phosphonate linkages.However, deprotection and purification techniques differ and a description of the procedures is included in the Technical Bulletin.We also offer the dC monomer with acetyl base protection.1This protecting group is removed with ammonium hydroxide during the cleavage step, eliminating modification at the dC sites during the deprotection step using ethylenediamine in ethanol.

Details

Usage

  • Coupling: 6 minutes. Note to prevent degradation of the methyl phosphonate linkage, low-water content oxidizer (40-4032) and DMAP for Cap B (40-4020) are recommended.
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_Me-Phosphonamidites.pdf).
Specifications
DiluentAnhydrous Acetonitrile
StorageRefrigerated storage, maximum of 2-8°C, dry
Stability24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions
LV40LV20040nm0.2μm1μm10μm
10-1100-020.25 g.25grams3.1190.3354.233.8824.6418.074.52
10-1100-050.5 g.5grams6.23194.33116.672.885338.879.72

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions
Molarity50nm0.2μm1μm15μm
10-1100-020.25 g.25grams4.650.0786.654.1339.365.41
10-1100-050.5 g.5grams9.290.07179.4112.1381.5511.21


品牌介绍
Glen research对我们的员工,我们的客户和环境的承诺Maravai LifeSciences致力于在所有业务活动中成为环境,健康与安全(EHS)管理方面的领导者,符合Maravai LifeSciences的商业利益和行为准则。我们承诺:雇员我们将通过提供零伤害的工作场所来预防伤害和疾病并促进健康的生活方式,从而对每位员工产生积极影响。我们将确保我们的员工具有执行此政策的意识,工具和知识。可持续发展我们将通过使用确保安全,效率和社会责任的商业惯例,以现在和将来保护我们的员工,社区,股东和环境的方式,进行可持续思考和采取行动。合规我们将遵守适用的法律和法规,同时提供程序和程序以确保我们的遵守。文化我们将通过停工授权,查明根本原因并采取纠正措施,倡导一种不容忍不安全状况和行为的文化。业务整合我们会将环境,健康和安全考虑因素纳入我们的业务活动。顾客我们将与客户紧密合作,以帮助他们满足其环境,健康和安全需求。供应商和承包商我们将与我们的供应商和承包商合作,以改善环境,健康和安全绩效。社区与政府我们将参与社区和政府的环境,健康和安全计划。这些价值观得到了Maravai高层管理人员的重视,并在组织内的所有责任级别得到强调。卡尔·赫尔(Carl Hull)首席执行官